If we could keep this board to single papers in single threads it will serve us best rather than intermixing other papers unless they are referenced or cited in paper posted. Thanks.
Re: Papers of Interest - > The Intercellular Communication Between Mesenchymal Stromal Cells and Hematopoietic Stem Cell
Posted: Fri Apr 01, 2022 3:18 am
The Intercellular Communication Between Mesenchymal Stromal Cells and Hematopoietic Stem Cells Critically Depends on NF-κB Signalling in the Mesenchymal Stromal Cells https://pubmed.ncbi.nlm.nih.gov/35347654/
Mesenchymal stromal cells (MSCs) regulate the fate of the hematopoietic stem cells (HSCs) through both cell-cell interactions and paracrine mechanisms involving multiple signalling pathways. We have previously shown that co-culturing of HSCs with CoCl2-treated MSCs expands functional HSCs. While performing these experiments, we had observed that the growth of CoCl2-treated MSCs was significantly stunted. Here, we show that CoCl2-treated MSCs possess activated NF-κB signalling pathway, and its pharmacological inhibition significantly relieves their growth arrest. Most interestingly, we found that pharmacological inhibition of NF-κB pathway in both control and CoCl2-treated MSCs completely blocks their intercellular communication with the co-cultured hematopoietic stem and progenitor cells (HSPCs), resulting in an extremely poor output of hematopoietic cells. Mechanistically, we show that this is due to the down-regulation of adhesion molecules and various HSC-supportive factors in the MSCs. This loss of physical interaction with HSPCs could be partially restored by treating the MSCs with calcium ionophore or calmodulin, suggesting that NF-κB regulates intracellular calcium flux in the MSCs. Importantly, the HSPCs co-cultured with NF-κB-inhibited-MSCs were in a quiescent state, which could be rescued by re-culturing them with untreated MSCs. Our data underscore a critical requirement of NF-κB signalling in the MSCs in intercellular communication between HSCs and MSCs for effective hematopoiesis to occur ex vivo. Our data raises a cautionary note against excessive use of anti-inflammatory drugs targeting NF-κB.
Re: Papers of Interest
Posted: Mon Apr 11, 2022 10:00 am
Inhaled Placental Mesenchymal Stromal Cell Secretome from Two- and Three-Dimensional Cell Cultures Promotes Survival and
Posted: Sun Apr 17, 2022 8:49 pm
Inhaled Placental Mesenchymal Stromal Cell Secretome from Two- and Three-Dimensional Cell Cultures Promotes Survival and Regeneration in Acute Lung Injury Model in Mice
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) is a common clinical problem, leading to significant morbidity and mortality, and no effective pharmacotherapy exists. The problem of ARDS causing mortality became more apparent during the COVID-19 pandemic. Biotherapeutic products containing multipotent mesenchymal stromal cell (MMSC) secretome may provide a new therapeutic paradigm for human healthcare due to their immunomodulating and regenerative abilities. The content and regenerative capacity of the secretome depends on cell origin and type of cultivation (two- or three-dimensional (2D/3D)). In this study, we investigated the proteomic profile of the secretome from 2D- and 3D-cultured placental MMSC and lung fibroblasts (LFBs) and the effect of inhalation of freeze-dried secretome on survival, lung inflammation, lung tissue regeneration, fibrin deposition in a lethal ALI model in mice. We found that three inhaled administrations of freeze-dried secretome from 2D- and 3D-cultured placental MMSC and LFB protected mice from death, restored the histological structure of damaged lungs, and decreased fibrin deposition. At the same time, 3D MMSC secretome exhibited a more pronounced trend in lung recovery than 2D MMSC and LFB-derived secretome in some measures. Taking together, these studies show that inhalation of cell secretome may also be considered as a potential therapy for the management of ARDS in patients suffering from severe pneumonia, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), however, their effectiveness requires further investigation.
Immortalized Mesenchymal Stem Cells: A Safe Cell Source for Cellular or Cell Membrane-Based Treatment of Glioma
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HEALTH AND SCIENCE CDC investigating 109 cases of severe hepatitis in kids across two dozen states, including 5 deaths
Posted: Sat May 07, 2022 10:49 am
HEALTH AND SCIENCE
CDC investigating 109 cases of severe hepatitis in kids across two dozen states, including 5 deaths
The Centers for Disease Control and Prevention is investigating 109 cases of severe hepatitis in children, including five deaths, the public health agency said on Friday.
More than 90% of the children were hospitalized and 14% required liver transplants, according to the CDC.
The public health agency has not yet determined a cause, but more than half the children had adenovirus infections.
The Centers for Disease Control and Prevention is investigating 109 cases of severe hepatitis in children, including five deaths, to determine a cause, with adenovirus infection as a primary line of inquiry, the public health agency said Friday.
More than 90% of the children were hospitalized and 14% required liver transplants, according to the CDC. The cases under investigation occurred over the past seven months across 25 states and territories. A majority of the patients have fully recovered and have been discharged from the hospital, according to the CDC.
Hepatitis is an inflammation of the liver that is often caused by viral infections, but environmental factors can also play a role. It is not uncommon in children but usually isn’t severe.